Detection of Toxoplasma gondii in Diabetic Patients Using the Nested PCR Assay via RE and B1 Genes

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detection of toxoplasma gondii in diabetic patients using the nested pcr assay via re and b1 genes

conclusions our study demonstrated that the b1 gene, more so than the re gene, showed positive samples and can be used to detect toxoplasmosis, although the b1 gene, in comparison to the re gene, did not show any superiority of molecular diagnosing capability. results also showed that toxoplasma molecular detection methods can be used instead of routine serological detection methods in a clinic...

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Detection of Toxoplasma gondii in Diabetic Patients Using the Nested PCR Assay via RE and B1 Genes.

BACKGROUND Toxoplasma gondii is an obligate intracellular protozoan parasite that exists worldwide. Various techniques have been developed for T. gondii detection. OBJECTIVES The aim of this study was the detection of T. gondii in diabetic patients with RE and B1 genes and the comparison of these two genes for diagnosis using the nested-PCR assay method. PATIENTS AND METHODS DNA samples fro...

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Detection of Toxoplasma gondii oocysts in soils in Northwestern China using a new semi-nested PCR assay

BACKGROUND Toxoplasma gondii is a zoonotic pathogen that can infect a range of animals and humans. Ingestion of T. gondii oocysts in soil is a significant transmission route for humans and animals acquiring toxoplasmosis. In the present study, we developed a new semi-nested PCR method to determine T. gondii oocysts distribution in soils in northwestern China. RESULTS The one tube semi-nested ...

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Detection Of Toxoplasma Gondii and Human Cytomegalovirus DNA in Blood from Transplant Recipients Using Multiplex Nested Polymerase Chain Reaction

Evidences from many studies suggested a polymerase chain reaction (PCR) as a valuable method for diagnosing infectious disease in the transplant recipients. We used this method for detection of Toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. DNA of both infectious agents were detected using two separate sets of nested pr...

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Serologic Detection of Anti Toxoplasma gondii Infection in Diabetic Patients

BACKGROUND Toxoplasmosis is caused by the Toxoplasma gondii parasite. The parasite is intracellular and can result in severe complications leading to death in immuno-deficient patients in particular. Diabetes is an important factor that increases susceptibility and risk of various infections in the host. OBJECTIVES The present study focused on the serologic detection of Toxoplasma gondii infe...

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ژورنال

عنوان ژورنال: Jundishapur Journal of Microbiology

سال: 2016

ISSN: 2008-3645,2008-4161

DOI: 10.5812/jjm.29493